spacer gif spacer gif spacer gif spacer gif Online submission spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 23 April 2008
doi: 10.1242/dev.017202

Development 135, 1913-1922 (2008)
Published by The Company of Biologists 2008
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Material
Right arrow All Versions of this Article:
dev.017202v1
135/11/1913    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Miura, G. I.
Right arrow Articles by Treisman, J. E.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miura, G. I.
Right arrow Articles by Treisman, J. E.

Myopic acts in the endocytic pathway to enhance signaling by the Drosophila EGF receptor

Grant I. Miura*, Jean-Yves Roignant, Michel Wassef{dagger} and Jessica E. Treisman{ddagger}

Kimmel Center for Biology and Medicine of the Skirball Institute, NYU School of Medicine, Department of Cell Biology, 540 First Avenue, New York, NY 10016, USA.

{ddagger} Author for correspondence (e-mail: treisman{at}saturn.med.nyu.edu)

Accepted 31 March 2008

Endocytosis of activated receptors can control signaling levels by exposing the receptors to novel downstream molecules or by instigating their degradation. Epidermal growth factor receptor (EGFR) signaling has crucial roles in development and is misregulated in many cancers. We report here that Myopic, the Drosophila homolog of the Bro1-domain tyrosine phosphatase HD-PTP, promotes EGFR signaling in vivo and in cultured cells. myopic is not required in the presence of activated Ras or in the absence of the ubiquitin ligase Cbl, indicating that it acts on internalized EGFR, and its overexpression enhances the activity of an activated form of EGFR. Myopic is localized to intracellular vesicles adjacent to Rab5-containing early endosomes, and its absence results in the enlargement of endosomal compartments. Loss of Myopic prevents cleavage of the EGFR cytoplasmic domain, a process controlled by the endocytic regulators Cbl and Sprouty. We suggest that Myopic promotes EGFR signaling by mediating its progression through the endocytic pathway.

Key words: ESCRT complex, MAP kinase, HD-PTP (PTPN23), Bro1 domain, Photoreceptor






© The Company of Biologists Ltd 2008