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Populations of NGF-dependent neurones differ in their requirement for BAX to undergo apoptosis in the absence of NGF/TrkA signalling in vivo

Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Studies, Summerhall Square, Edinburgh EH9 1QH, UK

View larger version (36K): [in a new window]   Fig. 1. The numbers of neurones with pyknotic nuclei and the total number of neurones in the SCG of wild-type, Ngf–/–, Bax–/– and Ngf–/– Bax–/– mice at E14, E18 and P1 in litters resulting from crossing Ngf+/– Bax+/– mice. The mean and s.e.m. of counts from both SCG of three to five embryos of each genotype at each age are shown.

View larger version (36K): [in a new window]   Fig. 2. The numbers of neurones with pyknotic nuclei and the total number of neurones in the SCG of wild-type, TrkA–/–, Bax–/– and TrkA–/– Bax–/– mice at E14, E18 and P1 in litters resulting from crossing TrkA+/– Bax+/– mice. The means and s.e.m. of counts from both SCG of three to five embryos of each genotype at each age are shown.

View larger version (34K): [in a new window]   Fig. 3. The numbers of neurones with pyknotic nuclei and the total number of neurones in the trigeminal ganglia of wild-type, Ngf–/–, Bax–/– and Ngf–/– Bax–/– mice at E14, E18 and P1 in litters resulting from crossing Ngf+/– Bax+/– mice. The means and s.e.m. of counts from both trigeminal ganglia of three to five embryos of each genotype at each age are shown.

View larger version (33K): [in a new window]   Fig. 4. The numbers of neurones with pyknotic nuclei and the total number of neurones in the SCG of wild-type, TrkA–/–, Bax–/– and TrkA–/– Bax–/– mice at E14, E18 and P1 in litters resulting from crossing TrkA+/– Bax+/– mice. The means and s.e.m. of counts from both trigeminal ganglia of three to five embryos of each genotype at each age are shown.

View larger version (24K): [in a new window]   Fig. 5. The cross sectional areas of the cell bodies of P1 SCG neurones (at the level of the nucleolus) of wild-type, Ngf–/–, Bax–/– and Ngf–/– Bax–/– mice resulting from crossing Ngf+/– Bax+/– mice and wild-type, TrkA–/–, Bax–/– and TrkA–/– Bax–/– mice resulting from crossing TrkA+/– Bax+/– mice. The percentage of neurones whose cross sectional area lies within defined limits are shown. The data are compiled from measurements derived from at least 100 neurones taken at random in the SCG of three neonates of each genotype.

View larger version (24K): [in a new window]   Fig. 6. The cross sectional areas of the cell bodies of P1 trigeminal neurones (at the level of the nucleolus) of wild-type, Ngf–/–, Bax–/– and Ngf–/– Bax–/– mice resulting from crossing Ngf+/– Bax+/– mice and wild-type, TrkA–/–, Bax–/– and TrkA–/– Bax–/– mice resulting from crossing TrkA+/– Bax+/– mice. The percentage of neurones whose cross sectional area lies within defined limits are shown. The data are compiled from measurements derived from at least 100 neurones taken at random in the trigeminal ganglia of three neonates of each genotype.

View larger version (32K): [in a new window]   Fig. 7. The mean and s.e.m. of measurements of the cross-sectional areas of the cell bodies of P1 SCG and trigeminal neurones (at the level of the nucleolus) of wild-type, Ngf–/–, Bax–/– and Ngf–/– Bax–/– mice resulting from crossing Ngf+/– Bax+/– mice and wild type, TrkA–/–, Bax–/– and TrkA–/– Bax–/– mice resulting from crossing TrkA+/– Bax+/– mice. The data are compiled from measurements derived from at least 100 neurones taken at random in the SCG and trigeminal ganglia of three neonates of each genotype.

View larger version (122K): [in a new window]   Fig. 8. Photomicrographs illustrating the typical appearance of P1 neurones in sections of the wild-type SCG (A), Ngf–/– Bax–/– SCG (B), wild-type trigeminal ganglia (D) and Ngf–/– Bax–/– trigeminal ganglia (E). The appearance of P1 Ngf–/– Bax–/– SCG (C) and trigeminal (F) neurones stained for ßIII tubulin is also shown. Because of the small size of the neurones in C, there are many neurones out of the plan of focus. Those within the plane of focus show the typical cytoplasmic staining for ßIII tubulin surrounding an unstained nuclear halo. Scale bar: 20 µm.

View larger version (17K): [in a new window]   Fig. 9. The percent survival of E18 SCG and E16 trigeminal neurones obtained from Bax–/– embryos grown for up to 5 days in defined medium without neurotrophic factors or medium containing 2 ng/ml NGF. The means and s.e.m. are shown (n=4 for each data point).

View larger version (170K): [in a new window]   Fig. 10. Photomicrographs illustrating the staining for BAX protein in sections of E14 (A) and P1 (B) trigeminal ganglia and E14 (C) and P1 (D) SCG of wild-type mice. Scale bar: 20 µm.