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Fig. 4. Effects of morpholino injections on the pattern of genes expressed in the
PSM. (A-G) The wild-type pattern, (H-N) embryos injected with
her1mo and (O-U) embryos injected with
her7mo. Two different morpholinos were used in each case
(see Materials and Methods), which caused identical effects, respectively. The
embryos are between the 8- and 12-somite stage. The columns represent the
different probes that were used for in situ hybridisation. her1ex
refers to a cDNA (exon) probe, her1in to the intron probe.
deltaC and deltaD are abbreviated. Two columns are shown for
the her1in and her7 probes, to show different stages of the dynamic
expression. Note that the her1in probe yields a much weaker signal
and requires about 15-fold longer staining times than the exon probe (1 hour
compared to approximately 15 hours). Moreover, it leads often to additional
apparently random punctate expression in the PSM, which is of unknown
significance. The embryos that are selected here have relatively few such
additional punctate signals - some of the other embryos in the same
preparations did show a higher number of these irregular signals. In total
185, 190, 88 and 84 her1mo-injected embryos from
independent experiments were hybridised with antisense riboprobes of her1,
her7, deltaC and deltaD, respectively. The penetrance of the
observed effects was 92%, 91%, 94% and 90%, respectively. In total 183, 163,
89 and 82 her7mo-injected embryos from independent
experiments were hybridised with antisense riboprobes of her1, her7,
deltaC and deltaD, respectively. The penetrance of the observed
effects was 96%, 92%, 91% and 91%, respectively. In total 230
her1mo-injected embryos were hybridised with her1
intron probe. The observed penetrance in 3 independent experiments was 92%,
96% and 93%, respectively. All in dorsal view, anterior to the top,
flat-mounted embryos.
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