Fig. 9. Neural convergent extension is required for elongation of the A/P axis. (A-D) Tor70 notochord staining of manipulated embryos. GFP grafted (A) and mock-operated (B; see Materials and Methods) embryos develop with normal A/P axes. Embryos with Xdsh-D2-expressing grafts into the neural plate (C) or with ablated posterior neural plates (D) develop with severe dorsal flexure. (E) Quantitation of notochord length in manipulated embryos, expressed relative to length of unmanipulated control embryos. Control, n=12; GFP-grafted, n=6; Xdsh-D2 grafted, n=10; mock, n=4; ablated, n=4. Data shown are mean±s.e.m.
