Fig. 1. Analysis of the expression conferred in vivo by the Fbp1 EcRU to the lacZ reporter transgene. The structures and names of the transgenic constructs are indicated on the left-hand side. Positions of the A (A) and enhancer (E) elements in the Fbp1 EcRU (-194 to –69 relative to the Fbp1 transcription start) are indicated. All constructs include the minimal Fbp1 promoter (-69 to +80) fused to the lacZ reporter gene. GATA-binding sites GBS1 (-130/-124), GBS2 (-90/-85) and GBS3 (-75/-70) are indicated with black boxes, EcR/USP-binding site EBS (-91/-103) is indicated by arrows. Late third instar larval tissues from transgenic lines with the indicated genotypes (right-hand side) were dissected and histochemically stained for determination of ß-galactosidase activity.
