Fig. 5. AEF-1 in third instar fat body nuclear extract binds to element A. Binding of proteins in a nuclear extract from late third instar fat body was analyzed in a gel shift assay using the element A (-194 to -138) as a probe in the presence or absence of competitor DNAs, AEF-1 antibody and protein A as indicated. C1, specific AEF-1 retarded complex; S1, AEF-1 supershifted complex; S2, nonspecific complex that formed when rabbit serum was incubated in the presence of fat body nuclear extract. The sequence of the element A probe and positions of the competitor DNAs are depicted at the bottom.
