Fig. 1. Phenotypic effects of EN-HTH^1-430. Phenotypes characteristic of dpp-Gal4/UAS-EN-HTH^1-430 flies grown at 24°C. (A-B) Ectopic eyes form in various positions on the ventral head cuticle, including the region of the maxillary palp (A) and the antennal foramen (B). (C,D) Hyperplasia of the eye is evident in adult flies (C) and eye imaginal discs dissected from a third instar larva (D). (E,F) Expression of EN-HTH^1-430 in the eye-antenna disc causes tufts of large bristles to appear in ectopic positions along the eye margin. (G,H) Cuticle preparations of antennae from dpp-Gal4/UAS-EN-HTH^1-430 flies showing an antenna-to-leg transformation. (G) The a2 antennal segment is relatively normal. The distal portion of the a3 segment is replaced by a leg-like structure. (H) Higher magnification of the transformed antenna reveals the presence of bracted bristles (arrows) typical of distal leg segments. (I,J) Cuticle preparations of a third (I) and second (J) leg of dpp-Gal4/UAS-EN-HTH^1-430 flies. The proximal leg segments are reduced in size and fused together (arrow in I). Distal parts of the truncated legs consist of disfigured leg structures with bristle patterns typical of tibia (arrow in J) or pretarsus (arrowheads in I and J). (K-L) Third leg discs from wild-type (K) or dpp-Gal4/UAS-EN-HTH^1-430 (L) third instar larvae shown at the same magnification. The EN-HTH^1-430 expressing disc is significantly enlarged and exhibits abnormal pattern of epithelial folds.

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