
Fig. 2. Phenotypic effects of VP16-HTH1-487. (A) Head of a dpp-Gal4/UAS-VP16-HTH1-487 fly exhibiting a complete loss of the eye. (B) Cuticle preparation of antennae from a dpp-Gal4/UAS-VP16-HTH1-487 fly. The a2 and a3 segments appear relatively normal, however the arista is either spineless and fused to the a3 segment (arrow) or completely missing. (C) A typical antenna of a dpp-Gal4/UAS-hth12 fly. The a3 segment is reduced in size and the arista is missing. (D-E) Cuticle preparations of a first leg (D) and a second leg (E) of a dpp-Gal4/UAS-VP16-HTH1-487 fly. C, coxa; Tr, trochanter; F, femur. The coxa, trochanter and proximal femur are unaffected. Distal leg structures are deformed and condensed together. The arrows indicate the claws. (F) First leg of a dpp-Gal4/UAS-hth12 fly. Proximal segments are intact, as they are in the leg in D. The leg is truncated and the remaining distal structures are malformed.