Fig. 3. VP16-HTH1-487 can rescue the loss of native HTH. The pattern of PNS neurons in stage 15-16 embryos as revealed by mAb 22C10 staining. In all panels anterior is to the left and dorsal is up. The boxed areas in A-C are shown in higher magnification in D-F; segment identity is indicated. (A,D) A heterozygous hth64-1 embryo showing a normal pattern of the PNS. The abdominal lateral chordotonal (LCh5) neurons can be clearly seen in the lateral PNS cluster of each abdominal segment A1-A7 (asterisks). (B,E) Similar views of a homozygous hth64-1 embryo. A loss of neurons and dorsal localization of the LCh5 neurons (indicated by arrows) are evident in all abdominal segments. (C,F) Expression of UAS-VP16-HTH1-487 was driven by Kr-Gal4 in a hth64-1 background. The LCh5 neurons are correctly positioned in abdominal segments A1-A4 (asterisks) where the transgene is expressed. More posterior segments do not exhibit such a rescue. Note the dorsal position of the LCh5 neurons in A5-A7 (arrows in C and F).
