Fig. 5. XMeis3 antisense morpholino oligonucleotide causes posterior truncations and anterior expansions in embryos. (A, top) Embryos at the one-cell stage were injected with 20 ng of the CMO. These embryos resembled uninjected controls. All embryos were fixed for photography at stage 38. (A, middle) Embryos at the one-cell stage were injected with 12.5 – 15 ng of the AMO. (A, bottom) Embryos at the one-cell stage were injected with 17.5-20 ng of the AMO. (B) Embryos from the experiment in A were grown to late neurula stages. Total RNA was also isolated from pools of seven embryos from each of the injected groups (lanes 1-6). RT-PCR analysis was performed with the markers: En2, Krox20 and HoxB9. EF1
served as a control for quantifying RNA levels in the different samples. For controls, RT-PCR (lane 2) and -RT-PCR (lane 1) were performed on total RNA isolated from control CMO injected embryos (lane 2). (C) Embryos at the one-cell stage were injected with 15 ng of the CMO (top) or the AMO (bottom). CMO- and AMO-injected embryos were co-injected with either 1.6 ng of XMeis3 RNA (middle) or 1.6 ng of hth RNA (right). White arrows mark the cement glands. All embryos were fixed for photography at stage 27/28.
