Fig. 4. Molecular analyses of the S-1-P lyase disruption mutant. (A) A schematic presentation of the parental gene, the disruption vector and the resultant mutant. (B) Southern analysis. Genomic DNA (20 µg) of wild-type Ax4 (W) and the S-1-P lyase null mutant (M) were digested with NdeI, ClaI and HindIII, separated on 0.8% agarose gel, and transferred to a nylon membrane. The membrane was probed with the sglA probe, stripped and then re-probed with the bsr probe. The asterisk represents a 0.1 kb ClaI fragment in which the insertion has occurred, to yield the 1.5 kb fragment. (C) Northern analysis of the S-1-P lyase disruption mutant. Total RNA samples (10 µg) of Ax4 and S-1-P lyase null cells were separated on 1% agarose/7.5% formamide gel, blotted onto nitrocellulose membrane and probed with sglA probe.
