Fig. 3. Leg patterning defects resulting from ectopic expression of fj. A,D,F, and G are adult female complete tarsi. E is an adult male leg showing the tarsus, tibia and femur. (A) Wild type. The lines indicate the joints separating the five tarsal segments. (B) fjN7. An incomplete joint is present at T2/3 (arrow). (C) HS-fj. An ectopic joint-like structure is present in the center of the T3 segment (arrow). (D-G) UAS-fj driven by: (D) 69B-Gal4, with truncations and loss of the T2/3 joint; (E) ptc-Gal4, with significant truncation and segment fusions of the tarsus. The arrowhead points to the juncture between the tarsus and the tibia. The sex comb is obvious on the tarsal remnant. (F,G) dpp-Gal4, with representative leg truncation and segment fusions, cuticular abnormalities, and outgrowths (arrows). The outgrowth in F appears to be segmented and both outgrowths contain bristles. The dpp-Gal4 stock alone has no detectable phenotypes (data not shown). Bar in A represents 50 µm in A,D,F, and G; 10 µm in B and C; and 100 µm in E.
