Fig. 7. Misexpression of hbs in imaginal discs leads to adult phenotypes. Distal wing margin in (A) wild type, (B) omb-GAL4/+;UAS-hbs/+ and (C) UAS-hbs/+;da-GAL4/+. The misexpression conditions lead to abnormal development of the wing margin (B) and notching (C). (D,E) The microchaetes on the scutum of wild type flies are linear (D, arrows) but disorganized on UAS-hbs/+;da-GAL4/+ flies (E, arrows). Wild-type flies show an orderly arrangement of eye structures (F,J,N), while in UAS-hbs/GMR-GAL4 (G,K,O), UAS-hbs/sca-GAL4 (H,L,P) and (I,M,Q) UAS-hbs/+;da-GAL4/+ flies, patterning is disrupted. Gain-of-function flies show rough eye phenotypes (G-I) and disorganization of ommatidia with anti-Elav staining (K-M). Fusion of ommatidia is evident as absence of pigment cells under darkfield microscopy (O-Q). Arrows in O-Q indicate examples of fused ommatidia.
