Fig. 3. mig-2, ced-10, and rac-2/3 redundantly and cell-autonomously control CAN axon pathfinding and cell migration. CAN axon pathfinding and cell migration were scored in living animals using ceh-23::gfp integrated transgenes (see Materials and Methods). The percentages of animals displaying defective posterior CAN axon pathfinding and defective CAN cell migration for each genotype are shown. The M+ designation in the genotypes indicates that animals had a wild-type maternal contribution of gene function. ‘+’ animals carried the kyIs8 integrated ceh-23::gfp transgene. lqEx27 is an extrachromosomal array bearing the unc-115 promoter::ced-10(G12V) cDNA transgene. lqEx53 is an array bearing the ceh-10 promoter::mig-2(+) cDNA transgene. lqEx56 is an array bearing the ceh-10 promoter::ced-10(+) cDNA transgene. Genotypes described as ‘sib’ represent the non-array-bearing siblings from the same brood as array-bearing animals. Error bars define 95% confidence intervals of the standard error of the proportion.
