Fig. 1. (A) Western blot analysis (using anti-Cripto antibody) of the conditioned medium of 293T cells transfected with cripto-His WT-, cripto-His Glu91Ala-expressing vectors, or vector alone. (B) Immunochemical analysis of Cripto-Fc chimera. Metabolically labelled conditioned medium from mock or cripto-Fc 293T transfectants was immunoprecipitated with protein A Sepharose and subjected to SDS-PAGE. The immunoreactive species were visualised after fluorography. (C) MZoep mutant embryos at sphere stage co-injected with recombinant Cripto-His protein and 10K dextran rhodamin. Fluorescence (a’,b’) and light (a,b) images of frontal (a,a’) and lateral (b,b’) views of injected MZoep mutant embryos. The molecular mass of protein standards is indicated (kDa).
