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Fig. 1. Expression of SER is established progressively. (A) In early third instar leg discs (~72 hours AEL), a ring of SER expression is detected in the coxa. (B) At ~78 hours AEL, a new ring of SER expression arises in the femur. (C) By ~84 hours AEL, SER expression is detected in four domains, two of which are in the tarsal region (ta2 and ta5). (D) By mid third instar (~96 hours AEL), SER is expressed in at least six prospective segments. It is unclear whether the new tarsal ring corresponds to ta1 or ta3; therefore, both the ta2 and the new ring are designated as ta. (E,F) Ultimately, by late third instar (~120 hours AEL) SER is expressed in one ring per segment and expression continues during pupal stages. co, coxa; fe, femur; ta2,tarsal segment 2; ta5, tarsal segment 5; ti, tibia.





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