Fig. 2. Expression of SER relative to the leg gap genes. SER expression (red) was compared with that of HTH, DAC, and DLL. (A,B) The coxa ring of SER (arrow) arises in cells proximal to those expressing DAC (A, green), within cells expressing HTH (B, green). SER expression is at the cell membrane and HTH expression is nuclear, so overlap in expression is not seen in all focal planes. Arrow in inset points to two cells obviously expressing both HTH and SER. (C-C’’’) Femur SER expression (bracket) arises within proximal DAC-expressing cells (blue). DLL expression (green) does not overlap with the femur SER expression. (D,E) At early-mid third instar, two sites of SER expression arise in the tarsus. A ring of expression (arrow) is observed distal to cells expressing DAC (D, green), but within cells expressing DLL (E, green). (F-F’’’) Expression of SER in the tibia (arrow) arises in cells expressing both DAC (blue) and DLL (green). DAC and DLL overlap in turquoise in C and F.
