Fig. 3. DAC positively regulates SER expression. (A-A’’) ptcGAL4 UASdac UASGFP results in a cell autonomous induction of SER expression (red, arrow) in early third instar leg discs. (B) ptcGAL4 UASdac adult leg with segmental fusions. fe, femur. (C-C’’) dac mutant clone showing a cell autonomous loss of SER expression in the femur (arrow). (E-E’) dac mutant clone in the tibia results in a cell autonomous loss of SER expression, except at the proximal edge of the clone (arrow). (F) dac homozygous mutant leg disc with greatly reduced SER expression in the femur (arrow). (D,G) dac mutant clones (outlined) cause segment fusions in adult legs. (D) Femur-tibia fusion. (G) Fusion extending from tarsal segments1-3. Arrows indicate where normal joints would lie (partially visible by upper arrow); asterisks denote apical bristles, which normally lie just proximal to tarsal segment borders.
