Fig. 6. DAC acts through minimal Ser enhancer elements. (A) Schematic of the Ser locus with genomic regions used for the lacZ reporter constructs indicated as SerlacZ2.2 (2.2 kb fragment) and SerlacZ1.9 (1.9 kb fragment) (Bachmann and Knust, 1998). (B-B’’) Expression of SerlacZ1.9 (red) is first detected in the prospective femur. ß-Galactosidase expression overlaps with DAC (blue) and lies distal to HTH (green). (C-C’’) FLP-out clones of dac (marked with GFP, green) result in cell autonomous induction of SerlacZ1.9 expression (red, arrow). (D) Expression of SerlacZ2.2 in tarsal segments 1-4 (green) is complementary to endogenous SER expression (red). (E,E’) FLP-out clones of dac (marked with GFP, green) result in cell autonomous repression of SerlacZ2.2 expression (red, arrow).
