Fig. 2. Overexpression of Ihh results in upregulation of Bmp genes. Forelimbs of wild-type (A-D) and ColII/Ihh mouse embryos (E-H) at stage E14.5 were sectioned and hybridized with riboprobes for Ihh (A,E), Bmp3 (B,F), Bmp4 (C,G) or Bmp7 (D,H). In limbs of ColII/Ihh mice, endogenous Ihh expression is reduced compared with that in the wild-type mice (A,E). Bmp3 (F), Bmp4 (G) and Bmp7 (H) are upregulated in perichondrium of ColII/Ihh mouse limbs compared with that of wild-type limbs (B-D). In addition, in ColII/Ihh transgenic mice Bmp4 (C,G) and Bmp7 (D,H) are upregulated in proliferating chondrocytes. All panels show sections through the radius.
