Fig. 4. EMS-induced gliolectin mutations affect commissure formation. Appearance of commissures stained with mAb BP102 in wild-type embryos (A) and in 3013 homozygotes (B) at mid-stage 13. Commissure formation is abnormal in the EMS mutant lines designated glec^m24 (C) and glec^m98 (D) but only when either is in combination with the deletion chromosome (3013) or with each other (E). Both the glec^m24/3013 and glec^m98/3013 embryos (C,D) are double-stained with BP102 in black and 1B7 (anti-Gliolectin) in brown, demonstrating that neither mutation abolishes the expression of the Gliolectin antigen. Incomplete commissure separation (B-E, arrowheads) is apparent in the deletion and in the EMS mutants, while decreased longitudinal density is less pronounced in the EMS mutants than in the deletion (B-E, arrows). Scale bar: 13 µm.

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