Fig. 2. Growth and hematopoietic potential of BCR/ABL-infected EB cells. (A) Methylcellulose colony formation of BCR/ABL-infected EB cells after 8 days on OP9 cells under several culture conditions. BL-MCM represents MCM containing SCF, VEGF and D4T-conditioned medium; Myelo-MCM represents MCM containing IL3, IL6, SCF and Epo; Base-MCM represents MCM without growth factors. (B) Growth of a representative liquid culture of BCR/ABL (bulk) and empty vector-infected EB cells in the presence of IL3, IL6 and SCF after 8 days on OP9 cells. (C) Colony formation of bulk cells after 30 days in liquid culture (without stroma). (D) Colony formation of clonal cells derived from BCR/ABL bulk cells. (E) Morphology of blast colonies (BL-CFC) (left) and corresponding cell populations (right). (F) Morphology of a primitive erythroid colony (EryP) (left) and corresponding nucleated primitive erythroid cells (right). Cytospins were stained with May-Grunwald-Giemsa. Experiments A, C and D were performed two, four and five times, respectively.
