Fig. 3. Engraftment of adult mice. Frequency of GFP-positive cells in bone marrow (BM), peripheral blood (PB) and spleen (Sp) of mice injected with bulk cells (A) or clonal cells (B). (C) Antibody staining profile of GFP-positive cells for Gr1/Mac1, Ter119, B220 and CD4/CD8 in BM, PB and Sp of mice injected with bulk cells. (D) Antibody staining profile of GFP-positive cells for Gr1/Mac1, Ter119, B220 and CD4/CD8 in BM, PB and Sp of mice injected with clonal cells. The number of mice engrafted and the total number of mice injected are indicated, respectively, in parentheses. (E) GFP-positive cells from peripheral blood were stained with lineage-specific antibodies, sorted, centrifuged onto glass slides and stained with May-Grunwald-Giemsa.
