Fig. 4. Flow cytometric analysis and globin gene expression of clones. (A) FACS-staining profile of clonal cells maintained in liquid culture in vitro. (B) RT-PCR analysis for adult (ß-major) and embryonic (ß-H1) globin mRNA from clones derived from BCR/ABL bulk cells (E1, E2 and E3) and sorted cells (GFP-positive Ter119-positive) from spleen (Sp) and bone marrow (BM) of mice injected with clones E1 and E2, respectively. A threefold dilution series for each sample confirmed that the PCR was in the linear range.
