Fig. 5. Hematopoietic colonies derived from the bone marrow (BM) of engrafted mice. Bone marrow from a primary engrafted animal was harvested and plated in MCM supplemented with growth factors (IL3, IL6, SCF and Epo). After 7-10 days, GFP-positive colonies were observed by fluorescence microscopy (A,C). Colonies were plucked, disrupted, centrifuged onto a glass slide, and stained to reveal hematopoietic morphology (B,D). (A,B) CFU-GEMM; (C,D) CFU-GM.

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