Fig. 6. Radioactive in situ hybridisation analysis of maxillary explants after 2 days in culture in the absence or presence of soluble human activin receptors IIA and IIB. Adjacent serial sections through presumptive molar regions were analysed as explained in Fig. 5. using bright field illumination to show histology (A,E,F,J,K,O), and dark field illumination to show mesenchymal Msx1 expression (B,G,L), and epithelial Irx1 (C,H,M) and Fst expression (D,I,N). Explants treated with 0 mg/ml (A-E), 12.5 mg/ml (F-J) and 25 mg/ml (K-O) soluble receptors are shown. Note that in order to assist the reader to assess Fst expression in the explants, dark field (D,I,N) and the corresponding bright field photomicrographs (E,J,O) of these samples are shown. Asterisks mark the same relative positions on adjacent serial sections.
