Fig. 6. Quantitative analysis of the effects of ectopic ephrin-A5 on muscle precursor cell numbers. Pax7-positive cells were counted, as described in the Materials and Methods. Ratios of cell numbers in ectopic ephrin-A5 limbs versus the contralateral limbs (pMES ephrin-A5) were compared with limbs expressing EGFP alone versus the contralateral limbs (pMES). Ratios were analyzed with the Statistical Analysis System general linear models procedure. The mean ratio for pMES ephrin-A5 is 0.53, whereas the mean ratio for pMES is 0.86. Ectopic expression of ephrin-A5 using pMES results in significant reductions in the mean numbers of muscle precursor cells in the forelimb (P=0.0008).
