Fig. 1. The phenotype of ale1 plants. (A-F) Gross morphology of aerial parts of plants. Plants were soil-grown in a covered container and the cover was removed 3 days after vernalization (DAV). (A) Wild-type plant, 7 DAV. (B,C) ale1-1 plants, 7 DAV. (D) Wild-type plant, 12 DAV. (E) ale1-1 plant, 12 DAV. (F) The same ale1-1 plant shown in E, at 16 DAV. The arrows in E and F indicate fused organs. (G,H) Transverse sections of wild-type (G) and ale1-1 (H) cotyledons. (I) Transverse section of fused leaves. (J,K) Adaxial surfaces of wild-type (J) and ale1-1 (K) cotyledons, 7 DAV. (L) Frequency of wrinkled leaves at each leaf position. Morphology of cotyledons and true leaves were observed 1 week and 3 week after vernalization, respectively. (M,N) Transmission electron micrographs of epidermal surfaces of wild-type (M) and ale1-1 (N) cotyledons. In the wild-type cotyledons, a densely staining layer covered the entire surface of each cotyledon. The arrowheads in M and N indicate the margin of the densely stained cuticle. cot, cotyledons; c, cuticle; cw, cell wall; cp, cytoplasm. Scale bars: 1 mm (A-F); 100 µm (G-I); 50 µm (J,K); and 1 µm (M,N).
