Fig. 5. Sequence alignment of Pelle with related protein kinase catalytic domains. Listed are the catalytic domains of D. melanogaster Pelle (gi|158046), Caenorhabditis elegans Pelle (gi|7505619), mouse IRAK-1 (sp|Q62406), Arabidopsis thaliana receptor kinase (gi|7488290), human FGF receptor 1 (gi|3114385, PDB: 1FGI) and human C-Jun N-terminal kinase (gi|5542282, PDB: 1JNK). Roman numerals indicate the positions of the 12 conserved subdomains found in all eukaryotic protein kinases. Red cylinders indicate alpha helices found in both the FGF-R and the JNK structure; red arrows indicate beta sheets present in both structures. Amino acids shown above the alignment indicate the substitutions found in pelle mutant alleles; pink indicates a loss-of-function allele, while red indicates a loss-of-function allele with antimorphic activity. In the alignment, residues in bold type are present in greater than 95% of kinases, residues in dark blue are present in greater than 70% of kinases, and residues in light blue are similar in greater than 50% of kinases. Residues in green are conserved in the Pelle family, but are uncommon (present in less than 15% of a broad sampling) in other eukaryotic protein kinases (Hanks and Hunter, 1995).
