Fig. 4. Supressor of Hairless (Su(H)) but not E(spl) is required in the follicle cells for exit from the mitotic cycle. (A) In this stage 12 egg chamber, Su(H)SF8 mutant cells (no green GFP) are smaller compared with the wild-type cells marked by nuclear GFP (green). Armadillo staining (red) outlines the cell boundaries while DAPI, blue, labels all cells. (B,C) In these stage 8 egg chambers, CycB (red) and PH3 (blue), indicate additional mitoses in Su(H)SF8 clones (arrows). (D) The Enhancer of Split (E(spl)) gene complex is not required in follicle cells to regulate the mitotic-to-endocycle transition, as no CycB or PH3 staining was observed in E(spl) mutant clones after stage 8 (mutant clones are black, arrows).
