Fig. 6. string expression in follicle cells. (A) The 
50 kb genomic region surrounding string is indicated. A thick black arrow indicates the string gene; the string intron is white. Stg15.3 and stg31.6 indicate the genomic fragments tested for rescue. Fragments used to drive lacZ expression in transgenic animals are shown below the genomic region. Cell types in ovary in which expression is driven by these fragments are described in Table 2. (B,D) The 4.9 kb element (D10) supports follicle cell expression in the germarium and stage 1-2 egg chambers. (C,E) The 6.4 kb element (D12) supports expression in stage 4-6 egg chambers. (B,C) X-gal staining, black arrows indicate the follicle cells with X-Gal staining, white arrows indicate lack of staining. (D,E) Anti-ß-gal (green). Yellow arrows indicate the follicle cells with staining, white arrows indicate lack of staining. (F) String is required for follicle cell mitotic divisions, as only 0-2 cell string mutant clones (black) were observed with 2-16 cell sister clones (bright green). On average, the mutant clone is one tenth the size of the sister clone. (H) Quantitation of the size of the string mutant (red bar) and sister (blue bar) clones. (G) Stg15.3 rescue construct shows partial rescue for the clone size (on average, the black mutant clone is half the size of the bright green sister clone). (I) Quantitation of the sizes of string clones with Stg15.3 rescue (red bar) and sister clones (blue bar). DAPI is blue in D,E and red in F,G. GFP is green in F,G. Mutant clones are indicated with unbroken white lines, sister clones with broken white lines. hhhhhfh
