Fig. 7. 6.4 kb string promoter region is precociously active in Delta germline clones. ß-Gal expression from 6.4-LacZ string promoter fusion (red) is observed in follicle cells of stage 5 (A) but not stage 9 (B) control egg chambers. Some overlapping expression is observed between CycB (blue) and the 6.4-LacZ string promoter fusion construct (red) at stage 5 egg chambers (A). In contrast to control egg chambers, in Delta germline clones (black germ line) the 6.4-LacZ string promoter fusion construct is still observed in the follicle cells at stage 8-9 egg chambers (C,D). Green in A-D shows GFP, which marks the wild-type cells, and cells that lack GFP expression are mutant clones (C is a fused egg chamber, the border between wild-type cyst and Delta germline clone is marked with arrows). Red in A-D shows ß-gal. Blue is CycB in A and DAPI in B-D.
