Fig. 2. Expression of GDNF in Pax2 mutants. (A) Whole-mount in situ hybridization of GDNF probe to E10.5 and E11.5 nephric duct (nd) and metanephric mesenchyme (m). In wild-type embryos, GDNF localizes to the posterior mesenchyme of the E10.5 urogenital region. Note the lack of GDNF staining in Pax2 mutants at both E10.5 and E11.5. (B) RT-PCR from RNAs isolated from E11.5 metanephric mesenchyme; genotypes are as indicated. Serial dilutions of total RNA was used for RT-PCR with GDNF specific primer pairs. Control RT-PCR reactions used primers for the Gapdh gene (GP3DH). Note lack of GDNF-specific PCR product in Pax2-/- mutants, but not in Ret-/- mutants.
