Fig. 5. Activation of GDNF mRNA in Pax2-expressing cell lines. (A) Northern blot analysis of parental 46m cells and Pax2 clonal derivatives probed for GDNF, Pax2 and GAPDH. Note increased GDNF mRNA in cells expressing Pax2b cell lines and low level of GDNF mRNA in Pax2a cell lines. (B) Infection of 46m cells with Pax2b expressing adenoviral vector. Top panel shows western blot of total cell lysates 24 hours after infection with increasing multiplicity, expressed as PFU/ml. Bottom panel shows a northern blot of uninfected 46m cells and cells infected with 2x109 PFU/ml at 8, 24 and 48 hours post infection. Note expression of GDNF mRNA in total RNA from infected cells.
