Fig. 8 Comparison of Pax2-binding sites. The PBS2 site from the GDNF 5' UTR was compared with known Pax2 DNA-binding sites. The previously described sites include a consensus sequence derived by PCR (Epstein et al., 1994); the consensus sequence from chromatin immunoprecipitation studies (Phelps and Dressler, 1996); the Pax2-binding site from the Pax5 enhancer (Pfeffer et al., 2000); and a Pax2 binding site from the WT1 promoter (McConnell et al., 1997). The alignment was made with the ClustalW feature from the MacVector DNA analysis software (Oxford Molecular Group). The PBS2 sequence corresponds to the position +768 to +807 of the published mouse 5' UTR (Tanaka et al., 2000). The region protected by the Pax2 paired domain is underlined. The gray boxes mark the consensus nucleotides for the set of sequences.
