Fig. 1. Expression of snail and worniu in early CNS. (A,B,E-H) RNA in situ hybridization of worniu. (C) RNA in situ of snail. (D) Antibody staining for Snail. (B) Double staining with Prospero antibody. (A-E) Wild-type embryos; the genotypes of the embryos in F-H are as indicated. The orientation of the embryos in this and following figures is anterior towards the left. (A-D) Lateral views; (E-H) Dorsal-ventral views. All the embryos are approximately at stage 9. wor RNA is expressed extensively in neuroblasts but in only a small number of GMCs, indicated by arrows (A). At a similar stage, many GMCs have formed, as indicated by the Prospero protein staining (arrows, B). (C,D) sna RNA and protein expression is also restricted predominantly to neuroblasts. (E-H) wor RNA expression is defective in embryos mutant for different pro-neural genes.
