Fig. 3. Olfactory bulb interneurons at E18.5 in wild types and Gsh2 mutants. DLX immunohistochemistry reveals positive cells in the SVZ, granule cell layer (GCL) and glomerular layer (GL) of the wild type (A). In the Gsh2 mutant (B) fewer than normal cells are positive for DLX and these cells are mostly confined to the SVZ. GABAergic interneurons of the granule cell layer and glomerular layer in the wild type (C) are revealed by immunohistochemistry for GAD67. In the Gsh2 mutant (D), there is a dramatic reduction in the number of GAD67 cells and most are found in the granule cell layer. E and F show the medial portion of the olfactory bulbs at higher magnification to visualize the few TH neurons present in the glomerular layer. Interneurons positive for TH are detected in the glomerular layer of wild-type (E) but not Gsh2 mutant (F) olfactory bulbs.
