Fig. 3. Genetic rescue of the lab mutant brain phenotype by transgenic expression of the Lab protein in a lab-null mutant background. Laser confocal microscopy of stage 15 embryos, reconstructions of optical sections. (A,C) Frontal views’ (B,D) lateral views. (A,B) From the same preparation; (C,D) from the same preparation. (A,B) Anti-HRP immunolabeling. Arrows indicate circumesophageal connectives, arrowhead indicates tritocerebral commissure. (C,D) Double immunolabeling with anti-HRP (red) and anti-Lab (green). Arrows indicate targeted misexpression domain of Lab in the lab mutant embryonic brain (equivalent to the endogeneous expression domain of Lab in the wild-type embryonic brain). Arrowhead indicates tritocerebral commissure.
