Fig. 4. Genetic rescue of the lab mutant brain phenotype by transgenic expression of the Scr protein (A-C) or the Antp protein (D-F) in a lab-null mutant background. Laser confocal microscopy of stage 15 embryos, reconstructions of optical sections. (A,D) Frontal views, (B,C,E,F) lateral views. (B,C) From the same preparation; (E,F) from the same preparation. (A,B,D,E) Anti-HRP immunolabeling. Arrows indicate circumesophageal connectives, arrowhead indicates tritocerebral commissure. (C) Double immunolabeling with anti-HRP (red) and anti-Scr (green). Arrow indicates targeted misexpression domain of Scr in the lab mutant embryonic brain (equivalent to the endogeneous expression domain of Lab in the wild-type embryonic brain). Asterisk labels the endogenous Scr expression domain in the subesophageal ganglion. (F) Double immunolabeling with anti-HRP (red) and anti-Antp (green). Arrow indicates targeted misexpression domain of Antp in the lab mutant embryonic brain (equivalent to the endogeneous expression domain of Lab in the wild-type embryonic brain). Asterisk labels the endogenous Antp expression domain in the subesophageal ganglion and ventral nerve cord.
