Fig. 3. Failure in regionalisation of the anterior neural plate in hOtx1²/hOtx1²; Gbx2^–/– embryos. (A-X) In situ hybridisation of sagittal sections of 9.7 d.p.c. wild-type (A-F), hOtx1²/hOtx1² (G-L), Gbx2^–/– (M-R) and hOtx1²/hOtx1²; Gbx2^–/– (S-X) embryos, with Otx2 (A,M), hOtx1 (G,S), Fgf8 (B,H,N,T), Pax2 (C,I,O,U), En1 (D,J,P,V), Wnt1 (E,K,Q,W) and Atx (F,L,R,X) probes reveals that in hOtx1²/hOtx1²; Gbx2^–/– embryos widespread co-expression of hOtx1, Fgf8, Pax2, En1 and Wnt1 is maintained and that the midbrain-specific gene Atx is not transcribed. Abbreviations as in previous figures. A',G',M',S' are bright-field images of the sections I A,G,M and S.

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