Fig. 6. RT-PCR transcriptional analyses of LHP1 (A) and CONSTANS (B) in the wild type and lhp1-1 mutant. (A) LHP1 expression is ubiquitous during development in wild-type plants whereas it is downregulated in the mutant. (B) CO expression is upregulated in lhp1 at an early vegetative stage. Expression of the constitutive APT1 gene was used as a positive control and to normalise the amounts of cDNA. Different organs or tissues were collected from plants grown in LD, at the following developmental stages: S-2c (two cotyledons), S-6rl (6 rosette leaves), S-eb (early bolting), S-of (first open flower). The tissues were WP (whole plants), AP (aerial parts), R (roots), FB (floral buds), F (mature flowers) and YS (young siliques).
