Fig. 1. Ectopic ß-galactosidase-containing cells in the ganglionic eminence of ngn2^lacZ/ngn2^lacZ mice. Sections of the telencephalon from ngn2^lacZKI mice at E14 (A-D,G) and E17 (E,F) showing the expression pattern of ß-galactosidase in blue. (A) ngn2^WT/ngn2^lacZ heterozygous, and (B) ngn2^lacZKI/ngn2^lacZKI homozygous littermates. The heterozygous mutant reveals a sharp boundary of X-gal staining between the positive cortex (CTX) and the negative ganglionic eminence (GE), whereas the homozygous mutant contains many ß-galactosidase-positive cells scattered in the GE. The arrow in B indicates the absence of ectopic cells in the ventricular zone (VZ) of the GE, in contrast to later stages (E17; F). CP indicates the cortical plate containing postmitotic neurons. (C) ngn2^WT/ngn2^lacZ heterozygous, and (D) ngn2^GFP/ngn2^lacZ homozygous mutant littermates. In the homozygous mutant carrying only one lacZ allele (D), a similar number of ectopic cells are present in the GE, as in the homozygote (B). (E) ngn2^WT/ngn2^lacZ heterozygous, and (F) ngn2^lacZ/ngn2^lacZ homozygous littermates at E17. The number of ectopic cells in the GE has increased compared to E14. The arrow in F indicates ectopic cells in the ventricular zone of the GE. (G) Higher magnification of the ectopic cells in the GE of ngn2^lacZ/ngn2^lacZ homozygous mutants at E14. The arrows indicate cells with morphologies characteristic of migratory neurons. Scale bar in A-F, 100 µm; in G, 25 µm.

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