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Fig. 5. Temporal expression of Sox1 and Gbx2 in EPL cell-derived neurectoderm. (A-F) Seeded EBM7 were cultured for a further 24 (A,D), 48 (B,E) and 72 (C,F) hours and analysed by in situ hybridisation with digoxigenin-labelled antisense probes directed against Sox1 (A-C) and Gbx2 (D-F). Aggregates were viewed using Hoffmann interference contrast microscopy. Size bar: 210 µm.





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