Fig. 7. fu interacts genetically with components of the Hedgehog pathway in the ovary. (A-C) Expression of a ptc-lacZ enhancer-trap in wild-type (A), hs-hh (B) or fu¹/fu^JB3; hs-hh/+ germaria. Ovarioles are oriented with anterior towards the left. (D,E) Removing one copy of cos2 (D) or two copies of Sufu (E) restores the fu ovarian phenotype. Class II fu alleles were not tested in these experiments because fu^II; cos2^WI/+ and fu^II; Su(fu)^LP flies die as late pupae, displaying a strong cos2 phenotype. fu^J denotes the fu^JB3 allele. (D) Females were grown at 25°C and dissected 5-8 days after eclosion. *indicates either cos2^WI or + (these two different genotypes were not distinguishable in our test). Flies came from the same cross for a given allele. (E) Females were grown at 21°C until late pupal stage, then shifted to 25°C and dissected 8 days after eclosion. Females originated from separate crosses performed in parallel. The total number of ovarioles examined for a given genotype was at least 150, except for fu¹ homozygous flies in E (n=39).

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