Fig. 5. Loss of Dephrin results in the abnormal exit of interneuronal axons from the CNS. We used a transformant line expressing tau-GFP to primarily label the projections of two interneurones (MP2 neurones, Gal4 line CY27). The axons of these neurones form a fascicle that extends in parallel on both sides of the ventral midline (dotted line; E,F). (A,B) Reducing the expression of Dephrin causes the interneuronal axons to exit the CNS (A, star) and severely disrupts the axonal scaffold (B, BP102). (C,D) DEph RNAi causes similar phenotypes as Dephrin RNAi. The interneuronal axons exit the CNS (C, star) or fasciculate loosely with each other. The axonal scaffold (D) is also severely disrupted. (E,F) Injection of dsCFP RNA rarely interferes with the projections of the MP2 neurones (E) or with the general layout of the axonal scaffold (F). Horizontal views of stage 17 embryos, anterior to the left.

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