Fig. 2. Somatic cyst cells do not correctly surround germ cells in stet mutant testes. (A) Diagram of a cross-section through the apical tip of a wild-type testis. Germ cells in blue, somatic cyst cells in yellow. Germline stem cells (S) and cyst progenitor cells (CP) next to the apical somatic hub (red); gonialblast (G), spermatogonia (SP) and somatic cyst cells (SC), as indicated. (B-G) Testes from (B,D,F) wild type and (C,E,G) stet mutants. Asterisks indicate apical tips. Scale bars: 0.1 mm. (B,C) Third instar larval (B) wild-type and (C) stet mutant testis tip stained with anti-FasIII (red) to label the hub and anti-Tj (green) to label cyst progenitor nuclei next to the hub (arrowheads). Cyst cell nuclei are displaced away from the hub (arrows). (D,E) Third instar larval wild-type and stet mutant testis tip stained for ß-galactosidase activity from the 17-en-40 marker. (D) Wild-type: cyst cells extend to surround the germ cells (arrows). (E) stet: ß-galactosidase is detected in dots (arrows) and small cytoplasmic extensions (arrowheads). (F,G) Adult wild-type and stet mutant testis tip expressing UAS-GFP under control of the ptc-GAL4 activator. (F) Wild-type: GFP is detected in extensions (arrows) surrounding the germ cells. (G) stet: GFP is mostly detected in dots (arrows) and a few extensions (arrowheads).

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