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Fig. 8. stet may function through Egfr signaling. (A-C) Phase contrast images. (A) stet mutant testis filled with small cells (arrows). (B,C) Spermatogenesis is restored in stet mutant testes expressing (B) a UAS-stet construct and (C) a UAS-rho construct in germ cells, note elongated sperm bundles (arrows). Occasionally, clusters of stet mutant germ cells can still be observed (arrowheads). (D,E) In situ hybridization to adult wild-type testes. (D) Star mRNA at high levels in germ cells at the apical tip (arrow). (E) Sense-control: no staining. (F,G) Immunocytochemical stain with anti-activated MAP-kinase (DP-ERK) to adult testes. (F) Wild-type: activated MAP-kinase in the somatic hub cells (arrow) and in the cytoplasm of cyst cells (arrowheads). (G) stet: activated MAP-kinase in somatic hub cells (arrow) and a few cyst cells (arrowhead) next to the hub. Scale bars: in A, 0.1 mm for A-C; in D, 0.1 mm for D,E; in F, 0.1 mm for F,G.





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