Fig. 2. Reduced expression of Fgfs and Dte and maintenance of midline tissues in Bmp4^tm1 mutants. Posteroventral views of wild-type embryos (A,D,G,I,K), and mutants (all other panels). (A-C) Fgf8 is expressed in the primitive streak of wild-type (A), but strongly reduced (n=5/6) in the posterior (arrowheads) of mutant embryos (B,C), at the five- to seven-somite stage. (D-F) Fgf4 is expressed in the primitive streak of wild-type (D) and one mutant (arrowhead; n=1/4) (F), but strongly reduced (arrowhead; n=3/4) in posterior of mutant embryos (E), at the one- to two-somite stage. (G-J) Shh is expressed in the node and midline of wild type at the headfold (G) to four-somite (I) stage, and mutant embryos at the headfold (H) to six-somite (J) stage (n=10/11). (K-M) Asymmetric Dte expression, enriched on right side of the node (arrowheads), in the wild-type (K) and one mutant (M; n=1/5), but low and patchy in the node (arrowheads) of other mutant embryos (L; n=4/5), at the four- to six-somite stage.

Return to article