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Fig. 4. Polar cells are required to specify centripetal cells. (A,B) fng mosaic follicles stained with Hoechst (DNA, white) and antibodies recognizing {pi}-MYC (fng+ cells, green), ß-galactosidase (BB127, centripetal cells, red) and FasIII (polar cells, cyan). (A) Stage 9 follicle with no fng+ anterior cells. No anterior polar cells form and no centripetal cells are specified (arrow in A). Arrowhead marks posterior polar cells. (B) Stage 10B follicle with only the two polar cells fng+ at the anterior (inset). The polar and centripetal cells are specified and properly migrate (arrow), even though all centripetal cells are fng-. Faint MYC stain in the anterior is germline expression.





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