Fig. 5. Polar cells are sufficient to specify border cells. Follicles with flip-out clones (marked with GFP, green) expressing constitutively activated Notch (ActN) and stained with Hoechst (DNA, white) and antibodies recognizing FasIII (polar cells, red). The arrows indicate the position of border cell groups. (A) Stage 9 follicle with an anterior group containing one polar cell and two outer border cells, and a posterior group containing two polar cells and six outer border cells. All cells express ActN. (B) Stage 10A follicle with an anterior group containing the only ActN cell, which behaves as a polar cell and migrates with three outer border cells. The posterior group includes the wild-type polar cells and five outer border cells. (C) Stage 9 follicle in which all cells express ActN and one group of border cells forms from four polar cells and 11 outer border cells.

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